1. Ultraviolet (UV) and Visible Spectroscopy: Introduction, Formation of absorption bands,
types of electronic transitions, Solvents effect on band position, Woodward-Fieser/Hoffman
rule for calculating λmax of alkenes, conjugated dienes, carbonyl compounds, benzene and its
derivatives. Chromophores, Kinetics study using UV-Vis spectroscopy, Electronic transition
for charge transfer complex, UPS and XPS.
2. Infrared Spectroscopy (IR) and Raman Spectroscopy: FT-IR, applications of IR
spectroscopy- interpretation of IR spectra-characterization of functional groups and
frequency shifts associated with structural changes-structural elucidation. Introduction of
2DIR spectroscopy.Raman spectroscopy and its application.
3. Nuclear Magnetic Resonance (NMR) Spectroscopy: Introduction, Instrumentation,
Relaxation process, chemical shifts-theory of NMR spectroscopy, Interaction of magnetic
field with nuclear spin, Lamour precession, resonance absorption of radiation, NMR
spectrometer, NMR spectrum, Shielding and deshielding of nuclei, Factors influencing
chemical shift, spin-spin coupling, factors affecting spin-spin coupling, spin-spin splitting-
complex, spin-spin splitting-first order, non-first order spectra, vicinal coupling; proton
exchange reactions; variable temperature spectra, the nuclear overhauser effect,
simplification of complex spectra, and introduction to 2D NMR techniques: COSY, NOESY;
Introduction of 13C NMR spectroscopy. Applications of NMR spectroscopy.
4. Mass Spectroscopy: Introduction, Techniques of ionizations: Electron impact, fast atom
bombardment, field desorption, matrix assisted laser desorption ionization (MALDI),
photoionization, resonance enhance multiphoton ionization (REMPI), thermal ionizations.
Principles of mass seperations, Mass analyzers: sector magnet technique. Quandrupole, time
of flight, Ion trap, Fourier Transform Ion cyclotron resonance. Sampling for mass
spectrometric measurements, Molecular beam, Supersonic expansion, Ionization potential
and measurements, Fragmentation of ions, rearrange of ions, base peak, isotopic peaks,
meta-stable ions, molecular mass determination, Detector for mass spectroscopy, mass
spectra of various classes of compounds and interpretation of mass spectra. Electrospray
Ionization Mass Spectrometry, Chemical Ionization mass s pectrometry, Time of flight
(TOF) mass spectrometry, MALDI-TOF mass spectrometry.
5. Time-Resolved spectroscopy: Introduction of time-resolved spectroscopy, basic principle of
LASER, working principles of some common LASER, Photoionization LASER
spectroscopy, spontaneous and stimulated emission: fluorescence and phosphorescence.
Ultrafast spectroscopy and dynamics.Time-resolved photoelectron spectroscopy. Tera-Hertz
time domain spectroscopy.
6. Structure-elucidation of inorganic and organic compounds by combined UV, IR, NMR and
Mass spectra